Continuity mistake: In the scene where the passengers board the plane at the beginning, the aircraft is a Boeing 737, noticeable by the crew jumpseats located by the entrance door. But the first scene you see where the aircraft is in flight, the aircraft changes to a Widebody Airbus, possibly an A310, noticeable by the different style of winglets that Airbus uses compared to that of Boeing. (00:02:15)
Continuity mistake: When Sam and Dean enter the Shoemaker home, a woman leaning against a wall holding a coffee cup starts to follow them down the hall. When the shot changes, the woman is back against the wall, talking to someone who wasn't there in the previous shot. (00:08:00)
Continuity mistake: In the scene where Jill is looking at her 'Bloody Mary' reflection, the lines of blood coming from her eyes go down and straight over her mouth (because she hasn't smeared them like the real Jill has). But as she begins to talk, the lines switch from being over her mouth to off to either side of her mouth and back multiple times.
Continuity mistake: During the scene where Sam is tied up and eventually fights the fake-Dean in Becka's house, there is a shot that shows both his feet and hands tied. During the fight, both his hands and feet are shown free, but he only cut free his hands, and began fighting immediately after that. (00:25:20 - 00:25:55)
Plot hole: When Sam and Dean realise they need to destroy the hook to stop the Hook Man, they find out that it has been reforged into something else. They state it needs to be set on fire to stop him, but if it was melted down and reforged then it has already been set on fire and the Hook Man shouldn't be latched on to it in the first place.
Continuity mistake: The baby gets into the fridge, which then closes - we see a closeup shot of the babyproofing clip shutting itself. Then when the mother sees the spilled milk and realises what's happened, there are a few shots of the fridge - in shots from the front the clip's open, and in shots from the side it's closed. When she goes to open the fridge it's open again - she even fiddles with it to give the appearance of undoing it before opening the fridge door.
Visible crew/equipment: When Sam tells the little girl to take her brother outside. The little girl runs out with her brother and Dean asks the little girl where Sam is, Dean runs to the Impala and grabs a shotgun. When Dean is running to the house, he runs on the lawn and you can see the shadow of the camera man running with Dean at the bottom of the screen.
Continuity mistake: When Dean reads about the Roosevelt Asylum in his dad's journal, there is a picture of a Wendigo on the opposite page of the newspaper clipping but in a different scene, Dean checks the article again for information on the South Wing in the Asylum but this time on the opposite page of the newspaper clipping, there is no picture.