Continuity mistake: When the victim falls to the ground in the pre-title sequence his eyes are closed and a pool of blood spills onto the floor from his mouth. When Tony and Ziva are examining the body and taking photo's of the victim, his eyes are now open and the pool of blood is gone from by his mouth. (00:00:50 - 00:03:10)
Continuity mistake: During The First Day's investigation of the double homicide Tony is wearing a grey suit, white shirt, and burgundy tie. On the second day, while Tony is wearing a dark suit, light blue shirt, and dark blue tie, when Gibbs tells Tony to go see what Ducky wants in Autopsy, Tony is back to wearing what he wore on The First Day. Then at Tobett's house, Tony is again wearing the light blue shirt and dark blue tie. (00:04:30 - 00:31:00)
Continuity mistake: When Tony & Ziva meet their contact in Naples, we see a local cop walking down the background behind them, and passing a walkway to the boat. Seconds later, as the camera changes view, we see that cop about 30 feet back, going the same way.