Continuity mistake: Inside the school lab, the Lizard walks to Peter on the floor and a stool next to him suddenly appears for him to pick up. (01:35:00)
Continuity mistake: When Peter is looking through his father's file in his bedroom, Uncle Ben comes in and starts playing with a Rubik's cube from Peter's desk. When he sets it down, the top and middle row are red and the bottom row is white. Shortly afterwards, when Peter is researching Doctor Connors on his computer, the top and middle rows are suddenly white and the bottom row is red (not fully solved, as another listed mistake claims).