Continuity mistake: During Andy's daydream at the beginning of the film, Mr. Potato Head has an eye patch over his left eye. When the daydream cuts back to reality we see that Andy has removed Mr. Potato Head's left eye to symbolize the eye patch. But when Andy's mum comes in with the video camera, his left eye is suddenly back in. (00:01:15 - 00:05:25)
Other mistake: After the magnet grabs Slink during the incinerator scene, all the toys grab metal objects to avoid getting torn up. Afterward, they all let go of their metal objects to drop down. However, Slink also drops down despite not holding on to anything because he is made of metal.
Deliberate mistake: Despite being squeeze toys, the Little Green Aliens couldn't possibly have got their feet stuck between the two dumpster lids because both the lids are closed after Big Baby throws Lotso in and the gap between the two dumpsters is far too small for any of the toys to get stuck between. Lotso certainly didn't lift the lid off-screen, we would have heard him do it.
Factual error: When Woody is looking at Andy's high school graduation picture in his room, there is a concert ticket in the upper left corner of the screen and another one just below it for a band called "Humble Beginnings" both with a date of Saturday, November 17, 2009. This date fell on a Tuesday. (00:11:15)
Continuity mistake: When Mom gives Molly the donation box she writes "Sunnyside" on one side of the corrugated box. When the Sunnyside box is in the car and also when Mom carries it to the daycare, there is printed writing at the side of the box (to the right of the "e" in Sunnyside) under the hand hole, but when Bonnie's Mom carries the box into the butterfly room that writing has vanished, then it reappears when the kids go to recess. (00:19:25)
Continuity mistake: In the scene where Woody is escaping from the daycare for the first time, we see him in a hallway hiding behind a no running yellow stand up sign. He is also at the hallway corner because we can see two women talking down the other side of the corner. He then proceeds down the hallway a couple of steps, he then seeing the janitor coming and immediately falls very close to that sign, Then from a bird eye view scene next, woody is now in the middle off the hallways and the sign is nowhere to be found as the janitor rolls over woody where the sign should be.
Continuity mistake: In the scene where the janitor goes into the bathroom to wash his hands, he originally, whilst entering the room pushing his cart complete in front of the stall, but when it cuts over to where woody sneaks into the stall, the cart is now moved to only halfway blocking the stall door.
Continuity mistake: When Woody and Buzz are standing on top of the cabinet looking at the photo they hear the kids and mom coming, they immediately jump down from the cabinet and land on the bottom open drawer, next to blue skateboard, then on the floor and they both run to the toy chest without looking back. Two shots later, when the door opens and the kids/mom come in, you can see that the drawer is now closed. (00:11:40)
Continuity mistake: After Chuckles tells the story of Lotso we see the gang locked up at the daycare center. Jessie comforts Bullseye and looks at the word "Andy" on her boot (with the "A" on the inner side) . Woody returns and when the bell rings for the kids to go outside, we see Jessie on the floor. Notice that the "Andy" on her boot is now different (the "A" is on the outer part of her boot). (00:52:50 - 00:57:10)
Plot hole: The first night the toys spent in the Daycare, when Buzz finds Lotso's band playing in the vending machine, there are no trucks patrolling the hallways, and the monkey does not sound the alarm when he sees that Buzz escaped. However, when Woody comes back later, he finds out that those security measures operate every single night.
Factual error: In the morning after Lotso incarcerates the toys, he bursts into the room in his truck. Just before he enters, we see the room's door handle turn downward and the door swings inward. There is no person or toy on the other side of the door turning that handle.
Continuity mistake: When the toys escape from the daycare, the paint on their bodies appear and disappear back and forth.
Continuity mistake: When Woody is escaping through the bathroom stall window, he climbs up the stall on the same side as the janitor's right shoulder, but he climbs out in the mirror image on the janitor's left shoulder. You can also see the smudge is on his left shoulder. He escaped on the opposite side of the stall to which he climbed up.