Revealing mistake: When Anakin and Obi-Wan are fighting on the big table, in the wide shot where Obi-Wan kicks Anakin for the second time, you can see that the one who performs the kick is not Ewan McGregor, but his stunt double. When Anakin lands on the table in the next shot, the one left standing is also McGregor's stunt double, and this time he is even wearing a brown bandanna that Obi-Wan does not wear. (01:51:15)
Continuity mistake: In the scene where Anakin arrives on Mustafar and tells R2-D2 to stay with the ship, he pulls the hood of his robe over his head with two organic hands. His right hand should be mechanical. [This mistake is mentioned in the audio commentary: this shot is taken from a shot of Obi-Wan later in the film when leaving Padme's apartment. Still a mistake, though.] (01:32:55)
Continuity mistake: During the Anakin/Obi-Wan fight scene Obi Wan switches lightsabers with Anakin. When Anakin is strangling him we can definitely see that Obi-Wan is holding Anakin's lightsaber in his right hand (note the hilt design), but when the shot is reversed Obi-Wan is holding his own lightsaber in that hand. (01:46:30)
Continuity mistake: When Anakin is in the medical centre lying on his back being transformed into Darth Vader, you can see the main part of the mask being lowered into place but Anakin is not wearing the neck section of the mask that it connects to. We then see the main part lowering directly downwards, in the next shot the neck section is in place. (02:01:20)
Other mistake: To escape from the elevator, Anakin cuts a hole in the ceiling. Later, when they rescue the Chancellor, they almost get crushed by another elevator (it is another one. Each elevator has a number. Obi Wan said the number of this elevator before and it was different than the one that had the hole). However, when a shot of this elevator is shown, it has the same hole that the other elevator has (better seen in slow motion).
Continuity mistake: When Palpatine is talking to Anakin in his office, there is a table at the end of the hall with a large object in the middle, and a gold cup on each side. When they leave the office and are talking in the hall, and a thin purple lamp/object appears on the end of it for a few shots, and vanishes whenever seen from then on.
Character mistake: During the opening space battle, Obi-Wan's ship falls under attack by buzz-droids, who proceed to cut into his fighter, disabling a number of systems and leading Anakin to try some decidedly dangerous moves to get rid of them. Both Anakin and Obi-Wan are acknowledged experts in the use of the Force and both have been shown to use the Force routinely, even for relatively mundane activities. It's also been firmly established that the Force works on droids and that the Force-user can still affect others through windows, viewscreens and so forth. Yet neither thinks to use the Force to deal with the buzz-droids. (00:05:10)
Continuity mistake: When Mace Windu and Palpatine fight, Windu backs Palpatine into the corner of the window. Note how close Windu is to Palpatine the whole time. Then from Anakin's perspective, Windu takes a few steps toward Palpatine, his saber pointed at Palpatine. When we saw it from the earlier perspective Windu never took those three steps toward a stationary Palpatine. (01:12:50)