Plot hole: At the beginning the Red Queen said that the last human settlement will fall in 48 hours unless Alice releases the anti-virus, however when Alice does release the anti-virus (a few seconds before the timer ends) she says it will take years for it to reach around all the corners of the globe, this does not make sense and implies that the last human settlement will fall anyway.
Trivia: While the film had the lowest domestic gross of the series ($26 million in comparison to the $40-$60 million each other film grossed), it was somewhat ironically also the highest grossing film of the franchise internationally, and as a result also ended up being the highest grossing film of the series worldwide, earning $312 million against a $40 million budget. It serves as a rare example of a film that is a flop in domestic markets while still technically being a resounding success.
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