Factual error: During the Nick Fury chase scene that takes place in D.C., several black and white state highway 6 signs are visible. These signs are unique to Ohio. Route 6 goes straight through downtown Cleveland where the scene was filmed. Plus during other chase scenes several Cleveland landmarks are visible in the background. (00:28:30)
Factual error: Just before they start to discuss the Mobile Launch Platform that has been hijacked in the Indian ocean, their GPS position is displayed on the bottom right corner of the screen INDIAN OCEAN Lat: 16N 55' 12.06" Long: 72N 56' 7.09 Longitude should be East/West, not North/South, plus the notation is incorrect. It should have been 16°55'12.06"N, 72°56'7.09"E. (00:03:43)
Continuity mistake: After the SHIELD agents have rescued the hostages, Steve begins a fight with one of the terrorists who tells him to take off his helmet. Steve takes it off and drops it beside him, leaving it on the floor. After Steve kicks the terrorist and runs over to him, you'll see in the next shot, that the helmet is still right beside him, despite his run. (00:11:35)
Continuity mistake: When Zola's algorithm is deployed and begins acquiring targets, we see a "grid" with the targets chosen. On the right, just above the pictures of the targets, there is a line indicating "targets acquired." Then we see the guns and then a counter indicating the amount of targets acquired, from 210,000 and up, but the next grid we see, in the "targets acquired" line says 133,213 and up. Besides, all the grids have the same pictures, but in different order.
Factual error: The location in DC has latitude and longitude both as N/S - longitude is an east/west factor. (00:13:05)
Character mistake: When Steve and Romanoff "switch on" Arnim Zola, Zola says "I may not be the man I was when the Captain took me prisoner in 1945." In the first Captain America, it was shown Steve took Zola into custody the same day as his friend Bucky supposedly died. However, the bio of Bucky shown at the Smithsonian earlier in the film said that he died in 1944. (01:00:25)
Continuity mistake: After the Winter Soldier blows up the car he assumed Romanoff was hiding behind, she sneaks up behind him and kicks the gun out of his hand. When he throws her at one of the cars, the gun is on the road to the left of some yellow lines, next to a car. A few shots later, when the Winter Soldier picks up the gun, the gun and car have both moved - the gun is now right on the lines, the car is still the same distance from it. (01:18:00)
Continuity mistake: In the scene where Nick Fury is showing Steve the new Helicarriers, just as Nick says "The satellites can read a terrorist's DNA before he steps outside the spider hole", the two are shown turning around. Nick is shown turning around almost completely and Steve is shown only turning half way. In the next shot, Nick is suddenly turned only half way, facing Steve. (00:16:20)
Factual error: When Rogers is launched through the front window of the bus after the Winter Soldier shoots him with a grenade, the bus is hit by a truck and flips. But if you look closely, the damage is minimal. On the truck it would qualify as a fender bender, and the dent in the bus looks like it was made with one decent swing from a sledgehammer. No way was that collision powerful enough to flip a fourteen ton bus on its side. (01:14:50)
Continuity mistake: When Steve and Romanoff are lying against the knocked over blinds after dodging the grenade, there is a box shown with some papers in it between them. The first shot shows a white piece of paper sticking furthest out of the box. Next shot, the furthest piece of paper sticking out of the box is now a peach coloured piece of paper. (00:12:45)
Continuity mistake: On the bridge when the Winter Soldier attacks; when Steve, Natasha, and Sam are in the car stopped, a Hummer rear ends them and starts to push the car. The first shot of the Winter Soldier shows various cars near him. Then a brief shot of Sam, and back to the Winter Soldier and the various cars have changed drastically.
Continuity mistake: When Steve and Natasha visit Sam in his apartment, in one scene, Natasha's hair is wavy. In the very next scene, her hair is pin straight.