Trivia: While this film is the fourth main-series entry, it is technically the eighth film in the overall franchise, as four direct-to-video spin-off movies were released between the third film (2003's "American Wedding") and this film. Eugene Levy is the only actor to appear in all eight films, and co-star Chris Owen is the only other actor besides Levy to appear in both the theatrical films (including a cameo in this entry) and one of the spin-offs. (With a role in the first spin-off "American Pie presents: Band Camp.").
Trivia: When Greg and Rowley approach the Cranium Shaker at the amusement park, the two people on the ride let out the Wilhelm scream.
Trivia: Before the One Percenter leader crashes his motor cycle into the chicken truck during the car chase sequence, the Wilhelm scream can be heard.
Trivia: When the fireball starts rolling inside the Mayan City, two soldiers fall off a cliff and one of them yells a Wilhelm scream.