Continuity mistake: When drunken Stewie is talking at the breakfast table to Brian, Brian has bacon and only one egg on his plate. After Stewie leaps from the shelf, Brian suddenly has two eggs.
Factual error: When Chicken Little is in his bedroom, after he scored during his baseball game, he looks into the spoon he is using as a microphone to see his reflection. He looks into the side that is curved away from you. When you look at that side of a spoon your reflection is upsidedown, but Chicken Little's was right side up.